UpToDate Iron: µg/dL. We compared Q-Plex results with reference-type assays and evaluated Q-Plex performance. Our objective was to determine prevalence of anaemia in an inpatient elderly population (≥60 years) and assess the usefulness of sTfR levels analyzed by other authors as a good predictor in the differential diagn… alternative.14 Red cell zinc protoporphyrin, soluble transferrin receptor, and the soluble transferrin receptor log ferritin ratio have been proposed as markers of functional iron deficiency. The Soluble Transferrin Receptor (sTfR) has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia (IDA) and anemia of chronic disease (ACD). It consists of a polypeptide strand with 2 N-glycosidically linked oligosaccharide chains and exists in numerous isoforms. Human sTfR (soluble Transferrin Receptor) ELISA Kit levels of ~10, 25 and 90 nmol/L of human soluble transferrin receptor in a buffered BSA matrix with human plasma and preservatives. Specimen collection and processing instructions for ... (2009) applied the method of Cook et al. Whole transferrin receptor (TfR) is present in reticulocyte exosomes. It exhibits a molecular mass of 85 kDa and forms a complex with transferrin in serum. The serum-soluble transferrin receptor (sTfR) is a truncated form of the previously described intact receptor, lacking the cytoplasmic and transmembrane domains [14]. Serum levels of the soluble transferrin receptor (sTfR) vary depending on the erythropoietic activity and iron status. 2020, … 2001;29(6):677-685. doi: 10.1016/S0301-472X(01)00641-5 PubMed Google Scholar Crossref Soluble transferrin receptor (sTfR) is cleaved from whole TfR in human plasma, with the remnant cytoplasmic domain (cTfR) remaining membrane associated. Soluble transferrin receptors are proteins found in blood that can be elevated with iron deficiency. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia. Anemia of CKD: Hypoproliferative, normocytic (usually), and … In humans, sTfR is a biomarker that can detect iron deficiency in the presence of inflammatory disease. Correspondingly, the soluble transferrin receptor-to-ferritin ratio was significantly decreased in groups 2, 3 and 4 (p<0.001). As a result, these sTFRs are released into the blood stream. Soluble transferrin receptors and iron deficiency 347 the endosomes (acidosomes), and the Tf-TfR complex is returned back to the cell surface, where apo-transferrin is released again [41]. High-fluorescence reticulocyte values of less than 8.3% showed 82% sensitivity and 30.2% specificity for recognizing iron-deficiency anemia patients. | Order Soluble Transferrin Receptor (sTfR) ELISA Kit (ABIN1058706). Following agglutination, the Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of iron-deficiency anemia.. It is usually not affected by chronic disease states, sTFR levels are about 6% higher in people in high altitudes (above 5200 ft/1600 m) and in African-Americans. Forest plot of the pooled effect size of the highest versus lowest soluble transferrin receptor for type 2 diabetes mellitus according to obesity (non-obese, mixed and obese participants). Human soluble transferrin receptor (sTfR) ELISA Kit Catalog Number. Data distribution (nontransformed) of soluble Transferrin Receptor (sTfR) concentrations (mg/L) at the four different sampling time points illustrated by bee-swarm box and whiskers plots Table 1. Transferrin is responsible for 50% to 70% of the iron binding capacity of serum. Serum soluble transferrin receptor concentration is an accurate estimate of the mass of tissue receptors Samir R’zik and Yves Beguin Department of Medicine, Division of Hematology, University of Liège, Liège, Belgium (Received 18 October 2000; revised 29 December 2000; accepted 24 January 2001) Objective. membrane receptor called the transferrin receptor (TfR; reviewed in references [1-3]). (2003) forestimatingbodyironasa derivative of ferritin and soluble transferrin receptor to an NHANES probability sample of non-pregnant women. Serum ferritin (SF), transferrin, soluble transferrin receptor (sTfR), and hemoglobin (Hb) were measured. Plasma membrane-bound transferrin receptor is released by proteolytic cleavage of the extracellular domain, resulting in the formation of a truncated soluble transferrin receptor (sTfR) that circulates freely in the blood. Blood testing of the soluble transferrin receptor (sTfR) is used as a measure of functional iron … Soluble transferrin receptor (sTfR) is a truncated form of the cellular transferrin receptor, without its transmembrane and cytoplasmic domains, and circulates bound to transferrin . An increased soluble transferrin receptor is helpful in diagnosing Iron Deficiency Anemia in a pt that also has Anemia of Chronic Disease. While the two tests (TIBC and transferrin) are different, they measure essentially the same thing and most laboratories only measure one or the other. UpToDate, electronic clinical resource tool for physicians and patients that provides information on Adult Primary Care and Internal Medicine, Allergy and Immunology, Cardiovascular Medicine, Emergency Medicine, Endocrinology and Diabetes, Family Medicine, Gastroenterology and Hepatology, Hematology, Infectious Diseases, Nephrology and Hypertension, Neurology, … Transferrin is a protein that attaches iron molecules and transports iron in the blood plasma. Thus, the concentration of sTfR in plasma or serum is an indirect measure of total TfR. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. Transferrin is a beta-globulin that carries iron through blood. Soluble Transferrin Receptor; The soluble transferrin receptor is rarely performed in clinical practice. When the number of complexes decrease, the number of vacant sTFRs increase. Cazzola M, Beguin Y, Bergamaschi G, et al. Soluble Transferrin Receptor (STR, sTfR): a quantitative measure of erythropoietic activity; in iron deficiency, cell membrane transferrin receptor density increases, and truncated forms of sTfR increase in the serum . Each vial contains 2.5 ml. Anemia of chronic disease (ACD), also known as anemia of inflammation (AI), is a functional anemia of iron-restricted erythropoiesis related to chronic diseases, such as infections, autoimmune diseases, cancer, and end-organ failure. Transferrin receptor is a transmembrane glycoprotein composed of two identical disulphide-linked subunits of 90 kDa, providing iron for cell proliferation and differentiation (2). Reagents for up to 96 tests. Pearson correlation and Lin’s concordance coefficients between the Q-Plex and reference assay were: … Adequate iron status at birth may prevent iron deficiency in early childhood. 21 Percent transferrin saturation was calculated as [iron/ (transferrin × 23)] × 100. Study: no study specified. membrane receptor, the transferrin receptor (TfR). OBJECTIVE —The relationships between iron metabolism and type 2 diabetes are bidirectional: iron affects glucose metabolism and glucose metabolism impinges on several iron metabolic pathways. We aimed to identify predictors of serum ferritin (SF) and serum soluble transferrin receptor (sTfR) in healthy newborns and to relate these iron indexes to iron status in 30248-9. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia. I couldn't find a consistent molar weight for transferrin, so you would need to use the molar mass of your material, and work it out from that. The concentration of soluble transferrin receptor (sTfR) in serum is reported to be useful in the diagnosis of iron deficiency, especially for patients with concurrent chronic disease, where routine tests of iron status are compromised by the inflammatory condition. In vitro, sTfR shed in the incubation medium correlates well with cellular TfR, but this relationship has never been established in vivo. The TfR is a mostly extracellular, transmembrane type II glycoprotein consisting of 2 identical polypeptides with a combined molecular weight of 180 kilodaltons. Br J Haematol 2001; 115:84. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of iron-deficiency anemia. 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